Gatewaytm bp clonasetm
WebTo perform the BP clonase reaction, select the pDEST17 and pDONR221 – DTU76545 PCR Product Entry clone sequences then go Cloning → Gateway Cloning. The Gateway cloning tool will identify the att sites … WebGateway™ Technology is schematically represented below. The attB × attP reaction is mediated by Gateway™ BP Clonase™ II enzyme mix; the attL × attR reaction is …
Gatewaytm bp clonasetm
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WebBP Clonase® II Enzyme Mix The BP Clonase® II enzyme mix (Box 2) contains the followin g rea ents. Store Box 2 at –20°C for up to 6 months. For long-term storage, store at –80°C. Item Composition Quantity BP Clonase® II Enzyme Mix Proprietary 40 μl Proteinase K solution 2 μg/μl in: 10 mM Tris-HCl, pH 7.5 20 mM CaCl 2 50% glycerol 40 μl WebPart Name: attB4: Description: BP Clonase TM, core recombination site for the Gateway(R) BP reaction, encoded by mutant version of attB: Source: GenSmart Design Collection: Publication: Size (bp) 21
WebJan 1, 2013 · Gateway Clonase Enzymes (back to Table of Contents) (back to Components) Name Size Catalog Number. Gateway BP Clonase Enzyme Mix 20 rxns 11789013. 100 rxns 11789021. BP clonase II enzyme mix (pre-mixed ready-to-use 20 rxns 11789020. solution of clonase and reaction buffer) 100 rxns 11789100. Gateway LR … WebDec 6, 2014 · BP clonase: 2 ul, 25 o C incubation for more than 24 hours. reaction was stopped by adding pronease k and incubation for 10 min at 37 o C. Cells used for …
The Gateway cloning method, invented and commercialized by Invitrogen since the late 1990s, is the cloning method of the integration and excision recombination reactions that take place when bacteriophage lambda infects bacteria. This technology provides a fast and highly efficient way to transport DNA sequences into multi-vector systems for functional analysis and protein expression using Gateway att sites, and two proprietary enzyme mixes called BP Clonase and LR Clonase…
WebMay 1, 2002 · The backbone of all described GATEWAY™-compatible vectors is the plasmid pPZP200 [. 1. ]. This plasmid is relatively small (6.7 kb), contains an origin for replication in E. coli (ColE1) and in Agrobacterium (pVS1), has a pBR322 bom site for mobilization from E. coli to Agrobacterium, and possesses a streptomycin and/or …
WebFeb 11, 2024 · by a BP reaction (GatewayTM BP ClonaseTM II Enzymemix, Invitrogen) to generate pENTR-BORIS with six C-terminal His-tags followed by a stop codon. To generate pI20-BORIS-6xH the entry clone was recombined into the lentiviral destination vector pI20 (empty vector (EV)-6xH, a kind gift from eibar firearmshttp://csrri.iit.edu/~howard/biol446/Lab1.bp_clonaseii_man.pdf follower leader dancing videoWebStore Gateway® BP Clonase™ II enzyme mix at -20ºC (non-frost-free freezer) for up to 6 months. For long-term storage, store at -80ºC. Components Supplied 20 rxns 100 rxns … follower leaderWebGateway® BP Clonase II Enzyme Mix Cat. No. 11789-020 Size: 20 reactions Cat. No. 11789-100 Size: 100 reactions Store at -20°C (non-frost-free freezer) Gateway® … follower liftWebGateway ® BP Clonase enzyme mix into an optimized single tube format to allow easier set-up of the BP recombination reaction. Use the protocol provided in this section to … eibar precision casting cifWeb5. Combine BP and LR reactions. When cloning a PCR product for Gateway expression you can save time and money if you skip the transformation and isolation steps after your BP clonase reaction to … eiba nottingham finalshttp://www.untergasser.de/lab/protocols/bp_gateway_reaction_ii_v1_0.htm ei bank business online