WebThe Agilent MitoXpress Xtra oxygen consumption assay allows real-time measurement of extracellular oxygen consumption of whole cells or isolated mitochondria. It is a reliable method for determining respiration rates for metabolic characterization and to evaluate toxic effects of treatments on mitochondrial function in a high-throughput format. WebE297 Extracellular OCR Plate Assay Kit. ... Extracellular OCR Plate Assay Kitには、培地中の酸素濃度が低下するとりん光強度が高くなる特性を持つOxygen Probeと、空気 …
ELISA Reader Machines & Equipment Dynex Technologies
Web4. Resuspend 300,000 cells/50 ul (6e6/ml) in desired XF assay buffer. a. (# cells * 50)/300k = amount to re-suspend cells in with appropriate media 5. Plate 50ul of cell suspension per well on coated plate and 50ul media in blank wells. 6. Spin plate for 1 min at 300g, set rotor to R2 accel/decel S/0. 7. WebAgilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. hedin nissan mölndal
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WebOverview. The Seahorse XF24-3 Extracellular Flux Analyzer allows for real-time quantitation of two major energy-yielding pathways in the cell: mitochondrial respiration and glycolysis. This instrument offers considerable improvements compared to former methodologies. XF24-3 analyzers do not require radiolabeled substrates, and unlike the ... WebNETosis Assay Kit (ab235979) provides a simple and fast method for studying the process of NETosis ex vivo. In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore (both included). Unbound neutrophil elastase is washed away following NET generation. WebKit will arrive packaged as a -20°C kit. After opening the kit, store individual components as stated below. Item Number Item Quantity/Size Storage 600801 Phosphorescent Oxygen Probe 1 vial -20°C 660910 HS Mineral Oil Assay Reagent 1 vial/15 ml Room temperature in the dark 600802 Cell-Based Assay Glucose Oxidase 1 vial/2 mg -20°C hedisa stuttgart